The structure and molecular basis of Cl -channels

Very few of the CP-channels described above have been purified, and the amino acid sequence is only known for the GABAA-receptor channel, the glycine-receptor channel and the Torpedo CP-channel [3-5], a CP-channel from skeletal muscle [125] and a CP-channel from MDCK cells [128]. In the following the current status will be briefly summarized. 

A very similar cloning strategy has been used for the 48-kDa subunit of the glycine-receptor channel by Betz and collaborators [4]. Strychnine was used as an affinity ligand, and synthetic oligopeptides were constructed from the purified material. They have been used to screen cDNA libraries. 

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