The single binding site

The single binding site for SM on the 30S ribosomal subunit is the 23S core particle of that subunit. A variety of reactions can follow from this event. One is the induction of conformational change in the complete 70S ribosome. Polyribosomes may break down, and the resulting monosomes could reassociate with mRNA, resulting in abnormal initiation complexes, thus inhibiting normal synthesis. 

The presence of two closely similar folding domains and the overall similarity to the riboflavin synthase fold has been confirmed recently by X-ray structure analysis. Despite that two-domain architecture, the fluorescent proteins bind only one ligand molecule per protein molecule, whereas riboflavin synthases have two ligand-binding sites per protomer. A recent mutagenesis study has located the single binding site to the N-terminal domain. ... 

When evaluating the sequences of the isolated clones it is import to carefully check whether no tandem repeats of the oligonucleotides were inserted. These would certainly be more active than the single-binding sites... 

The enzyme is made of four identical subunits each having a single binding site for each ligand. 

High yields are possible due to the large capacity of the ChiraLig for the single enantiomer on each load cycle. A significant percentage of the available binding sites are used in each cycle to bind the eutomer. 

Outline of the experiment to determine the orientation of a single binding site on unphosphorylated Il ... 

One of the earliest explanations to be considered was that n molecules of ligand might bind simultaneously to a single binding site, R ... 

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