The Quantitative Determination of DNA Oxidation Products

A method has been reported for the quantification of the DNA oxidation products, 8-hydroxy-2,-deoxyguanosine (8-OH-dG), 8-hydroxy-2 -deoxyadenosine (8-OH-dA), 5-hydroxymethyl-2-deoxyuridine (HMDU), thymidine glycol (TG) and 2-hydroxy-2 -deoxyadcnosinc (2-OH-dA) [37], The HPLC system employed consisted of a 2.0 x 250 mm Qs column and gradient elution from watcrmcthanol, (94 6) to (10 90) over 28 min, at a flow rate of 200 ptlmin-1. 

It may seem obvious but it is always worthwhile checking that the ions/decom-positions selected for monitoring are sensible with respect to the structure of the analyte(s) involved rather than waste time developing a method based on ions from a background component. 

The need for choosing appropriate ions/decompositions to provide adequate sensitivity and selectivity has been stressed and the selectivity provided by mass 

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