The Kiss

Kiss-Roll Colters. In kiss-roU coating, the web passes over a roU wet with the coating fluid, and has no backing roU. There are many types of kiss-roU coaters. The kiss roU can turn in the dkection of the web or in the reverse dkection but usually operates in the web dkection. Kiss-roU coaters are tension sensitive and are often used to apply excess coating prior to a metering device. 

It is very important right from the start to bear in mind the current axiom offered in the KISS principal, keep it simple stupid. While this may seem a little offensive, nonetheless, from time to time, one sees systems that gi the impression the originators went out of their way to make things unnecessarily complicated. An example of this is controls put on systems that with a little added thought could have been made self-regulating. 

Rinard dedicated his research to a detailed analysis of methodological aspects of a micro-reactor plant concept which he also termed mini-plant production [85] (see also [4, 9, 10] for a commented, short description). Important criteria in this concept are JIT (Just-in-time) production, zero holdup, inherent safety, modularity and the KISS (keep it simple, stupid) principle. Based on this conceptual definition, Rinard describes different phases in plant development. Essential for his entire work is the pragmatic way of finding process solutions, truly of hybrid character ]149] (miniaturization only where really needed). Recent investigations are concerned with the scalability of hybrid micro-reactor plants and the limits thereof ]149], Expliddy he recommends jointly using micro- and meso-scale components. 

Oh, Mistress Ysa He s hard, you see, with all the kissing and the touching. And—and it hurts a little. That s your maidenhead breaking. There s blood like when you get your monthlies, only just a little if he s kind, and I m sure such a fine gentleman as Master... 

I smiled, though the tears were still cold in the corners of my eyes. He kissed me on the mouth and I clung to him, so that the kiss went on even to the quickening of my desire, and his. All cleverness, all cozening had drained from me with my tears. He moved to the middle of the bed and drew me with him. For a moment my cheek touched his pillow it was still wet with his own weeping. 

However, an alternative pathway that bypasses clathrin-mediated endocytosis and EEs appears to be available as well. This model of endocytosis known as kiss and run or its variant kiss and stay have attracted increasing interest in recent years [74] (Fig. 9-9B). Kiss and run has been directly demonstrated with dense-core granules in neuroendocrine cells [84, 85], and this model would explain some observations that are not readily accommodated by the classical pathway. The kiss and run model proposes that neurotransmitters are released by a transient fusion pore, rather than by a complete fusion with integration of the synaptic vesicle components into the plasma membrane. Synaptic membrane proteins never lose their association and the vesicle reforms when the pore closes. As a result, the empty vesicle can be refilled and reused without going through clathrin-mediated endocytosis and sorting in the EEs. 

Direct demonstrations of the kiss and run model in mammalian synapses have remained elusive [73], but there is sufficient evidence to suggest that there may be up... 

Now, in procession, the Great Companions of the Table approach and, as you give to each one the kiss of peace, they are absorbed into you, taking their places within, until only the Regent remains. He will remain in the Turret Room and minister to you there. 

I blew her a kiss and went around the comer to the kitchen door. My key worked, but the door would not open. Then I heard bolts being drawn I was admitted. We completed the kiss in proper form, ignoring Mlana s smiles in the background. 

Clearly the 4-substituent is important, and the 5-substituent calls the shots (as with the 4-substituent in the phenethylamines). But is it possible that anything at the six-position is the kiss of death ... 

In the kiss-and-run mode exocytosis and endocytosis are directly coupled to each other, while in the case of classical complete vesicle fusion, exocytosis and slow clathrin-mediated endocytosis are timely and spatially separated. However, it appears that also in the latter case exocytosis and endocytosis occur coordinated, as both are stimulated by an increase of the cytoplasmic calcium concentration. It has been shown that after calcium entry the enzyme phospho-inositol-5 kinase Iy, which is enriched in the synapse, catalyzes the synthesis of phosphatidylinos-itol (4,5)-bisphosphate and that this mechanism is important for synaptic vesicle trafficking (Di Paolo et al. 2004). As many proteins involved in clathrin-mediated endocytosis are recruited to the plasma membrane by binding to phosphatidylinosi-tol (4,5)-bisphosphate (e.g., amphiphysin, dynamin, epsin, AP-180, and AP-2) it is attractive to speculate that elevated levels of calcium mediate the recruitment of en-docytic proteins to the plasma membrane by this mechanism. The increased level of phosphatidylinositol (4,5)-bisphosphate could be in part degraded by synaptojanin that thereby initiates the disassembly of the clathrin coat. Hence, calcium-induced transient increases in the level of phosphatidylinositol (4,5)-bisphosphate appear to play a central role for coupling exocytosis to clathrin-mediated endocytosis. In addition, it has been demonstrated that calcium also leads to the dephosphorylation of endocytic proteins as amphiphysin, dynamin, and synaptojanin, which in vitro is important for efficient coat assembly (Cousin and Robinson 2001). 

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