The general status of mass spectrometry in peptide analysis

9 The general status of mass spectrometry in peptide analysis 

Why was MS slow to gain favour in peptide structure determination After all, by 1965, when the early mass spectra of peptides were being collected and assessed, sophisticated examples of structure determination by MS were commonplace in other areas of the organic chemistry of natural products. 

Although the interpretation of peptide mass spectra is relatively straightforward, the problem of low volatility - getting enough of the peptide sample into the gas phase to give satisfactory mass spectra - dissuaded many early potential users of electron-impact ionisation MS (EIMS the only routine technique for mass 

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