The Double-Isotope Dansyl Assay

The principle of the double-isotope dansyl assay is as follows A sample containing ammo acids m unknown quantities is added to a mixture of C-labeled ammo acids, and the resulting mixture is dansylated using [ H]-dansyl chloride. Following separation of the dansylated amino acids, generally by thin-layer chromatography, the ratio dpm [ H].dpm ( C) for each dansyl ammo acid is measured. This ratio bears a linear relation to the amount of unlabeled amino acid present m the original sample see section 2.4). 

The Double-Isotope Dansyl Microassay for Measurement of Cerebral Amino Acids 

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Example Protocol Using the Double-Isotope Dansyl Assay... 

The double isotope dansyl assay has been used extensively to measure amino acids in nervous tissue. In Table 1, glutamate concentrations in regions of the adult rat CNS are compared using the technique described in section 2.5 3 Data is taken from the published study of Butterworth et al., 1979. 

Advantages of the double-isotope dansyl assay may be summarized as follows ... 

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