The development of new MISPE protocols

Three different steps are involved in the development of a new SPE protocol based on molecular imprints the synthesis of the material, the assessment of its 

The assessment can also be done by an off-line SPE procedure where the cumulative recovery curves of the sorbents are evaluated in different solvents and at different pHs. A suitability test for propranolol imprinted sorbents, to be used in SPE of plasma and urine samples, was developed by Olsen et al. [34]. This was based on the solvent composition required to give a recovery of either 20 or 50% of the extracted material (ES20 or ES50 values) on the blank and the imprinted cartridge. The method was developed in order to allow a rapid estimate of the specific and non-specific contributions to overall binding. 

Finally a validation step for the overall MISPE procedure is mandatory to allow the use of the method itself in place of the regulatory methods. The issues to be considered here are the accuracy and precision of the method based on the MISPE protocol, its limit of quantification, selectivity and ruggedness. In particular the inter- and intra-assay precision need to be checked with real samples and certified reference materials and methods [23,24]. 

---