The Conformation of Sialyl Lewis Bound to E-selectin

Examples will illustrate the capabilities of the methodology described above. No attempt was made to review the field in total since several very good review articles have appeared during the recent years [54-56]. 

The precise distribution of the two orientations and the presence of other local minima in aqueous solution is still a matter of dispute. Upon binding to E-selectin only one of the two major conformational families generated by different orientations of the a-D-Neu5Ac-(2— 3)-P-D Gal hnkage is recogniz. The bound conformation is characterized by the observation of a trNOE between H3-Gal and H8-Neu5Ac, whereas no interaction is observed between H3-Gal and H3ax- 

Since usually conformations that are recognized by proteins are preformed in aqueous solution we reinvestigated the solution conformation of sLe and Le to find out whether this non-exo-anomeric conformation is also present in aqueous solution. The presence of this minimum had been predicted recently on the basis of 

7 Interaction of Bacterial Lipopolysaccharide Fragments with Monoclonal Antibodies 

---