The Anabolism of Pyrimidine Nucleotides

The overall scheme of pyrimidine nucleotide biosynthesis differs from that of purine nucleotides in that the pyrimidine ring is assembled before it is attached to ribose-5-phosphate. The carbon and nitrogen atoms of the pyrimidine ring come from carbamoyl phosphate and aspartate. The production of carbamoyl phosphate for pyrimidine biosynthesis takes place in the cytosol, and the nitrogen donor is glutamine. (We already saw a reaction for the production of carbamoyl phosphate when we discussed the urea cycle in Section 23.6. That reaction differs from this one because it takes place in mitochondria and the nitrogen donor is NH/). 

The reaction of carbamoyl phosphate with aspartate to produce W-carbamo-ylaspartate is the committed step in pyrimidine biosynthesis. The compounds involved in reactions up to this point in the pathway can play other roles in metabolism after this point, A -carbamoylaspartate can be used only to produce pyrimidines—thus the term committed step. This reaction is catalyzed by aspartate transcarbamoylase, which we discussed in detail in Ghapter 7 as a prime example of an allosteric enzyme subject to feedback regulation. The next step, the conversion of A-carbamoylaspartate to dihydroorotate, takes place in a reaction that involves an intramolecular dehydration (loss of water) as well as cyclization. This reaction is catalyzed by dihydroorotase. Dihydroorotate is converted to orotate by dihydroorotate dehydrogenase, with the concomitant conversion of NAD to NADH. A pyrimidine nucleotide is now formed by the reaction of orotate with PRPP to give orotidine-5 -monophosphate (OMP), which is a reaction similar to the one that takes place in purine salvage (Section 23.8). Orotate phosphoribosyltransferase catalyzes this reaction. Finally, orotidine-5 -phosphate decarboxylase catalyzes the conversion of OMP to UMP 

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