The Ampholine Carrier Ampholytes

High density—must give a minimum density difference of 0.12 g/cm . 

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The sample, the Ampholine carrier ampholytes and the sucrose solution are mixed by means of a gradient mixer or manually (see Section VI,5,6). The compartment (7) is filled so as to achieve a density gradient. Filling is done via nipple (2), using a funnel or pump. The second electrode solution is added on top of the solution to be electrofocused. This electrode solution consists of distilled water and acid or base, depending on whether the upper electrode is to be the anode or the cathode. 

This mixture is made up to 30 ml with water. The Ampholine carrier ampholytes are not added until the gel has polymerized and been thoroughly washed with water. 

Figure 30. Pattern obtained by the technique of Dale and Latner using 1 jvl normal serum. The original electrofocusing gel cylinder can be seen horizontally in the upper part. The Ampholine carrier ampholytes can be observed as a long black shadow to the right in the picture, where they ready have left the electrophoresis zone. For explanation of the pattern, see Fig. 31 (Dale and Latner, 34).

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