Tetraploids progeny

Tai, G. C. C., De Jong, H. (1997). A comparison of performance of tetraploid progenies produced by diploid and their vegetatively doubled (tetraploid) couterpart parents. Theoretical and Applied Genetics, 94, 303-308. 

Tetraploid broodstock offers the possibility to produce triploid (3n) progenies directly through tetraploid (4n) x diploid (2n) crosses. As such progenies will consist of 100% or close to 100% triploid individuals without the need for chemical treatment, they offer advantages over the chemical induction method in terms of efficiency, ease of crossing procedure and safety. 

Ploidy levels of the resulting progenies were mixed, as in any chemical induction, because embryonic development is not uniform between oocytes and thus different ploidy levels were achieved. Pair matings also showed different results in terms of representation of ploidy classes, indicating parental influences. A difference in size between ploidy levels, as observed in oyster by McCombie et al (2005a), was used to concentrate tetraploid offspring into pseudocohorts as they developed. 

MCCOMBIE H, LAPEGUE s, coRNETTE F, LEDU c and BOUDRY p (2005b) Chromosome loss in bi-parental progenies of tetraploid Pacific oyster Crassostrea gigas. Aquaculture 247, 97-105. 

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