Tetranoic acid

Holman Greenberg (1953) reported the content of polyenoic acids of lamb, swine and beef determined by alkaline isomerization and ultra-violet spectrophometry. In testis of lamb 15.6% of the fatty acids were hexaenoic and 10.3% were tetraenoic in testis of hog 1.5% were hexaenoic and 8.8% were tetraenoic in testis of beef 5.8% were tetranoic acids. Later, indirect evidence was obtained by Cole (1956) suggesting the presence of large amounts of highly unsaturated fatty acids in the testis of the golden hamster. 

Holman, R.T., The ratio of trienoic tetranoic acids in tissue lipids as a measure of essential fatty acid requirement, J. Nutr., 70, 405-410, 1960. 

Some reduction of tetranoic fatty acids of the n-6 family (20 4 and 22 4) and of 22 6 n-3, and the prominent well known rise of 20 3 n-9 are observed, in the animals raised on the fat free diet. 

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