Terms and Methods of Enzyme Kinetics

2 TERMS AND METHODS OF ENZYME KINETICS The simplest picture of an enzymic reaction is given by 

If a steady-state approximation for [ES] is used (see Section 2.2) instead of the rapid-equilibrium assumption, then one obtains Eq. (8.4), known as the Briggs-Haldane equation  

This equation has the same mathematical form as Eq. (8.2), except that ( 2 The will be the same as defined previously only if 

If it is assumed that the substrate binding and proton equilibria are rapidly maintained, then the initial rate can be put in the standard Michaelis-Menten form  

If the conditions are such that [S] (called swamping conditions), then the variation of with pH can be used to determine K . On the other hand, if [S], then is independent of ATj and die values of K i and /sT j can be determined. 

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