Termination of Escherichia coli Chromosomal Replication

Initiation and Termination of Escherichia coli Chromosomal Replication 

Two aspects of E. coli chromosomal replication still to be considered are initiation and termination. From what has been said we conjecture that replication initiates at a unique site, proceeds bidirectionally, and terminates at a point where the two oppositely advancing growth forks meet. To study initiation it was first necessary to isolate that segment 

With circular minichromosomes containing this unique segment termed oriC, it has been possible to study the initiation and replication process in vitro. First, in crude extracts it was possible to show that replication initiates within or near the oriC sequence and proceeds bidirectionally. About 13 different proteins participate in the oriC-directed DNA replication as judged by their activity in a reconstituted replication reaction. 

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Replication of the Escherichia coli Chromosome Initiation and Termination of Escherichia coli Chromosomal Replication DNA Replication in Eukaryotic Cells Eukaryotic Chromosomal DNA SV40 Is Similar to Its Host in Its Mode of Replication... 

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