Tentative unique genes

Fig. 3.1 EST representation in functional categories, BlastX and TeraBlastX searches were used to assign putative identities to tentative unique genes (TUGs) from I. pini, Bombyx mori, and Callosohruchus macuialus. Putatitve identities were based on the best hit, or, if the best hit was an un-annotated sequence e.g., from a genome project), then a lower, but still confident hit was used to assign function. The cut-off E value was set at 10. ...

Fig. 3.2 Classification of "metabolism ESTs. Tentative unique genes (TUGs) from each species that clustered into the metabolism category (see Fig. 3.1J were further subdivided. Here, carbohydrate incorporates all glycoside hydrolases, lipid includes lipases, B-oxidation and steroid metabolizing enzymes, protein includes predominantly proteases and their precursors, and amino acid includes all enzymes involved in the interconversion of amino acids. Esterase includes those esterase-like enzymes with unknown substrates, and other contains those TUGs that do not sort into other categories. These include predominantly oxidoreductases and purine/pyrimidine metabolizing enzymes.

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