Tentacle-type

Fig. 11a. Fractionation of (/) chymotrypsinogen A, (2) cytochrome C and (3) lysozyme on strong cation exchangers, a) Support Fractogel TSK 650(s)SP (conventional type) sample, 1 mg each flow rate, 1 ml/min column size, 150 x 10 mm T.D. Solvent A = 0.02 mol/1 phosphate, pH 6.0 solvent B = A + 1 mol/1 NaCl gradient, 0-10 min, 0% B 10-70 min, 0-100% B. b) Support Fractogel EMD 650(s)SO( — (tentacle type) conditions as in (a) [78]...

Compared with conventional type, the tentacle type exhibits a marked increase in selectivity. The results observed for preparative ion exchangers of tentacle type prompted the author to test this structural arrangement of the binding groups also for analytical materials based on porous silica. Figure 12 reveals that the tentacle-specific selectivity is fully preserved when the matrix is changed. 

Tentacle-type modification 165, 166 Tetanus antigen 144 Tetraethylenepentamine (TEPA) 150 Tetrafluoroethylene 161 Thrombin 145 Trains 148... 

Weitzhandler, M., Farnan, D., Horvath, J., Rohrer, J. S., Slingsby, R. W., Avdal-ovic, N., and Pohl, C., Protein variant separations by cation-exchange chromatography on tentacle-type polymeric stationary phases, /. Chromatogr. A, 828, 365, 1998. 

A further polymeric modification is the so-called tentacle type (Fig. 3.14c), where the polymer or oligomer does not encapsulate the bead. This stationary phase is useful for mild separation or purification of proteins by ion-exchange chromatography, as described by Muller (1986, 1990). Chang et al. (1985) and Chang and An (1988) reported a hydrophobic interaction chromatography (HIC) variant where long, flexible side-chains can surround the proteins and thereby interact only with their hydro-... 

Figure 3.11 Presentation of ionic ligands on different ion-exchange type reins, (a) directly attached to the surface (b) tentacle type ion-exchange resin (c) enhanced flexibility tentacle resins.

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