Temperature isotachophoresis

Fanali et al. have described a capillary isotachophoresis method for the determination of procaine in pharmaceuticals [ 150]. The drug was determined in a 6 pL sample of solution (Spofa product, obtained from Czechoslovakia, and diluted 180-fold) by cationic isotachophoresis in the single column mode. The system used a PTFE capillary column (20 cm x 0.3 mm) and a conductivity detector. The separation was carried out at room temperature, at 50 pA (but switched to 25 pA during detection). 

In the development of the method itself, the increase in the temperature in the isotachophoretic zones played an important role since temperature measurement was the first universal detection procedure with sufficient sensitivity in the capillary isotachophoresis . In connection with the detection by means of a thermocouple, more attention was devoted to the study of interrelations between the longitudinal distribution of the temperature on the outside wall of the isotachophoretic column and the width and the position of the zone boundary inside the column. At present, the thermocouple detector lost greater significance however, the temperature regime in the isotachophoretic column still requires adequate attention. 

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