Techniques for Preparation of Meiotic Chromosomes

Two basic procedures have been developed for studies on mammalian chromosomes during the last decade the squash technique and the airdrying method. Both are direct adaptations to testicular material of similar methods used for somatic tissues. 

From 1956 28) 1964, the squash techniques were widely used for 

A testis is removed, placed in hypotonic sodium citrate solution (0.7% wt/vol) at room temperature, and swirled in the solution to remove adherent fat. 

The tunica is incised, and the mass of tubules is transferred into 

The tubules are transferred into fixative (50% glacial acetic acid). 

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