Target-specific complexes

Figure 22.17 Antibody-liposome conjugates may be used as targeting reagents for detection or therapeutic applications. The liposome may be constructed to contain fluorescent molecules for detection purposes or bioactive agents for therapy. The antibody component targets the complex for binding to specific antigenic determinants.

The method(s), supporting validation reports, related knowledge and target specifications are critical inputs into risk assessment process. A review is performed jointly by a transfer team from both sites. There is an assessment of complexity (simple vs. complex) and prior knowledge (similar methods/API/products already transferred vs. no experience), method robustness (low vs. high concern), which can affect quality post transfer (low vs. high risk). 

In the context of this chapter, biomimetic is defined as the nse of simple synthetic media to mimic a complex biological process . Earlier Fendler (1984) defined membrane biomimetic chemistry as processes in simple media that mimic aspects of biomembranes . Thus, classical biomimetic approaches target specific... 

Prakash, S., and Prakash, L. Specific complex formation between yeast RAD6 and RAD18 proteins a potential mechanism for targeting RAD6 ubiquitin-conjugating activity to DNA damage sites. Genes Dev. 1994, 8, 811-20. 

The target (I column in Table 15.1) is also described by the structural complexity nitric S and size metric H (4 and 5 columns), as defined in Chapters 5 and 11 (Whitlock 1998). A more complex molecule does not necessarily require a more complex synthesis, although it is often so, particularly wten the specific complexity S/H is high and the starting materials are simple molecules. 

Fig. 2.17. Degradation of the tumor suppressor protein p53 by the ubiquitin-proteosome system. The oncoprotein E6 of the human papilloma virus (HPV) forms a specific complex with the p53 protein and can thus induce the degradation of p53. The E6-p53 complex is recognized by E6-AP, a E3 enzyme of the ubiquitin pathway, as a target protein, whereby a ubiquitin residue is transferred to a lysine residue of p53. In this process, the E6 protein serves as the recognition element for ubiquitin hgation of p53.

In conclusion, the ProxiMol technique may enable us to form the maps of multiprotein complexes as it provides both targeting specific cell surface receptors and other proteins associated with this receptor. No matter what technique is used to form phage libraries, it is not possible to interpret the data manually it is accomplished by using bioinformatics. 

---