Target site identification

The three components of a chemical genetic process to uncover novel sites of pesticide action, chemical libraries, phenotype screens and target site identification, can be organized and deployed in a variety of different ways and with varying degrees of complexity, depending on project objectives. 

Chemical Genetics, Target Site Identification, Resistant Mutants, Herbicide Mode of Action, Auxins, Arahidopsis... 

The OPCW Laboratory performs functional tests on the authenticated, certified, and on-site analytical databases. These are tests that verify that the analytical database is functional. So far, this procedure is fully developed for the MS analytical databases. The electronic version of OCAD (which so far consists of MS analytical data only) is tested with the NIST MS Search/Analysis program. The on-site MS analytical databases are tested using AMDIS software. A NIST or AMDIS library is created and this becomes the target library for a library search. A search is performed on a newly measured MS spectrum (raw data files) containing a number of chemicals that are present in the target library. Identification of the targeted spectrum indicates that the created library is functional. 

Figure 2-12 Simplified overview of the mechanism of action of SMlD-based SM target profiling and protein SM binding site identification.

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