Target proteins targets

Regulation by relocation. Mechanisms to overcome cellular compartmentalization are pivotal points of regulatory control. Relocation may involve targeting proteins. Targeting subunits guide both protein kinases and phosphatases to their substrates, located in different subcellular compartments. 

Compound Effector molecule Targeting protein Target Application Clinical status References... 

Moleeular biology eloning expression isotope labeling of protein target... 

Water-soluble polymers and polyelectrolytes (e.g., polyethylene glycol, polyethylene imine polyacrylic acid) have been used success-hilly in protein precipitations, and there has been some success in affinity precipitations wherein appropriate ligands attached to polymers can couple with the target proteins to enhance their aggregation. Protein precipitation can also be achieved using pH adjustment, since proteins generally exhibit their lowest solubility at their isoelectric point. Temperature variations at constant salt concentration allow for frac tional precipitation of proteins. 

Target protein is not stable in the chosen buffer system for SEC... 

Target protein may have been proreolyzed Nonspecific interaction... 

FIGURE 16.17 Structure of chyinotrypsiu (white) ill a complex with egliii C (blue ribbon structure), a target protein. The residues of the catalytic triad (His ", Asp , and Ser ) are highlighted. His (blue) is flanked above by Aspios on the right by Ser (yel-... 

FIGURE 23.22 The metabolic effects of insulin. As described in Chapter 34, binding of insulin to membrane receptors stimulates the protein kinase activity of the receptor. Subsequent phosphorylation of target proteins modulates the effects indicated. 

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