Tandem MS with FT-ICR Instruments - Part II

The mechanism of peptide fragmentation upon ECD is still a matter of debate [152-154]. In particular, there is a discussion as to whether the ECD process is nonergodic (so fast that there is no internal equilibration of energy prior to dissociation) [145,147] or ergodic (following QET) [154]. 

Note Peptide fragments formed after transfer of a hydrogen (atom) are marked by a prime after the letter, e.g., the fragments from ECD are c and z ions [152]. 

As obvious from the preceding pages, both IRMPD and ECD are intimately connected with FT-ICR instrumentation. Now having considered these methods for ion activation in some detail we may focus our attention on their practical realization on the FT-ICR platform and some illustrative applications. 

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