Tail clip test

The A -demethylation pathway is common to the metabolism of Ml 25 (Vllf) and morphine, both drugs being converted to secondary amines by liver micro-somes under the same condition [55]. Reaction rates were depressed when microsomes from rats repeatedly treated with Ml25 or morphine were used. In the same work, rats were-shown to become tolerant to the pharmacological effects of M125 (shock avoidance and tail clip tests) and cross tolerance to morphine was also found. 

However, to improve reproducibility for forelimb grip strength we recommend orienting the force transducer vertically (Columbus Instruments, Columbus, OH, USA) and modifying the test procedure as follows. A weight (100 g) is attached to the base of the mouse s tail using a small plastic clip. The mouse is held by the scruff of the neck with the tail and weight in the... 

Wash the recidue left on the filter paper with 60% alcohol to remove excess ammonium acetate. To ensure this add a pinch of solid NH Cl to the recidue on the filter paper and wash with alcohol till the filtrate is free from chloride (as tested with silver nitrate solution, the filtrate is perfectly clear when free from chloride). If the washing is to be interrupted such as for the night, attach a rubber tube to the tail of the fimnel and pinch it tight with a clip when there is solution above the level of soil in the filter paper, i.e. in no case the soil should dry otherwise loss of ammonia may occur. 

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