T- Mineral Acid

When using chloramine T-mineral acid reagents care should be taken to treat the layer with chloramine T first and then with mineral acid. The sensitivity is considerably reduced if the plate is, for example, merely dipped once in a combined reagent consisting of chloramine T in 5 percent sulfuric acid. 

Care must be exercised in the choice of acid employed in chloramine T — mineral acid reagent since the detection sensitivity and also the color of the fluorescences produced depend to a significant extent on the choice of acid. This is illustrated for the purine derivatives caffeine, theobromine and theophylline in Figure 1 and Table 1. 

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