Synthetic Nucleic Acids SNA

Another approach may be to use an in vitro repHcation system [347]. But in vitro replication eliminates many of the benefits and the built-up familiarity with cells. However, regardless of the organism used, at some point, one will want to 

In general, the approach would be almost like a cloning process where only the action of DNA polymerase is allowed during cell division but the role of RNA polymerase is eliminated. To achieve the previous goals, the authors used a SNA in vivo. SNA will be used here to represent one member from the entire family of nucleic acid analogs. The desired properties for the SNA include the following  

1) The SNA must be able to be easily replicated and maintained in cells. This means that the cloning strain must contain a DNA polymerase such as an enzyme that can faithfully replicate SNA to produce more SNA. 

2) The SNA must not be recognized by RNA polymerase, as SNA inserts should not be expressed. 

3) The SNA should be easily convertible to and from DNA to allow compatibility with other techniques. 

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