Synthetic Genetics

The synthetic genetic system has even been coupled to unnatural protein synthesis. Adding extra letters to the genetic alphabet has been shown to increase the number of triplet codons that are accessible to a messenger RNA. In 1993, Bain et al. showed that additional triplet codons made possible by extra nucleobases and delivered by synthetic biological efforts could encode extra amino acids.14 That result was obtained with the terran ribosome. 

Cai Y, Hartnett B, Gustafsson C, Peccoud J (2007) A syntactic model to design and verify synthetic genetic constructs derived from standard biological parts. Bioinformatics 23 2760-2767... 

We repeat these simple examples from earlier work since they form a major justification for the elaborations we report below, and if they are understood well, then much of what follows should be clear. The dynamics in the differential equations are in qualitative agreement with the dynamics in the synthetic genetic networks in E. coU. One important aspect of biology is to understand the ways in which the organization and structure of the control networks can be used to predict the dynamics. Thus, we would like to develop methods that could be used to predict the dynamic behaviors just demonstrated without integrating or carrying out the stability analysis of the differential equations. 

SYNTHETIC GENETIC METHODS TO DETERMINE PROTEIN INTERACTIONS... 

V. (2011) pBAMl an all-synthetic genetic tool for analysis and construction of complex bacterial phenotypes. BMC MicrabioL, 11, 38. 

Yang Z, Chen F, Alvarado JB, Benner SA (2011) Amplification, mutation, and sequencing of a six-letter synthetic genetic system. J Am Chem Soc 133(38) 15105-15112. doi 10.1021/ja204910n... 

Pinheiro VB, Taylor AI, Cozens C, Abramov M, Renders M et al (2012) Synthetic genetic polymers capable of heredity and evolutimi. Scitmce 336 341-344... 

Tabor JJ, Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte EM, Voigt CA, Ellington AD. Department of Pharmaceutical Chemistry, School of Pharmacy, University of California San Francisco, San Francisco, CA 94158, USA. A synthetic genetic edge detection program. Cell. 2009 Jun 26 137(7) 1272-81. 

Here, we present a complementary chemical biology strategy that applies the logic of synthetic genetic interaction. 

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