Synthesis of 3-HP from Glycerol

Deletion of the fabi gene can also be appUed in other malonyl-CoA based pathways, such as pathway 8, in order to increase the malonyl-CoA pool for 3-HP production. This malonyl-CoA mediated pathway was also used for the development of novel metabolic pathways for the production of P(3HB-co-3HP) from a structurally unrelated carbon source in Cupriavidus necator, in which malonyl-CoA reductase and the 3-HP-CoA synthetase domain of propionyl-CoA synthase from C. aurantiacus were employed [36]. Recombinant C. necator with malonyl-CoA mediated 3-HP synthetic activity could produce poly(3-hydroxybutyrate-co-3-hydroxypropionate) (P(3HB-co-3HP)), in which 3-HP monomer could be incorporated into copolymer up to 2.1 mol%, from fructose or even alkanoic acids [36]. 

Crude glycerol is a cheap substrate, a readily available waste byproduct of current biodiesel production processes. Its potential as a carbon source for microbial fermentation in the production of industrially important chemicals is currently being exploited [37, 38]. Glycerol is a reduced, three-carbon compound like 3-HP, so the reaction steps for the conversion of glycerol into 3-HP are generally 

In the reductive pathway, 3-HPA, the main intermediate of 3-HP production, can be produced from glycerol by introduction of CoA-independent dha and CoA-dependent pdu regulons. 

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Synthesis of 3-HP from Glycerol Through the CoA-Dependent Pathway... 

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