Synergi Hydro

Separation of the amino acids is achieved on a Synergy Hydro-RP column (250x 4.6 mm, 4-pm particles Phenomenex 00G-4375-E0) with a 20 x4.0 mm 08-DB guard column (Supelco 59565). 

Figure 8-25. pH study of zwitterionic compound A on a Phenomenex Synergy-Hydro-RP column. Method conditions are indicated in the figure. 

Fig. 1 Dependence of k on adrenaline (squares) and L-tyrosine hydrazide (circles), on mobile-phase concentration of 1-hexane-sulfonate. Column Synergi Hydro-RP (Phenomenex) 150 x 4.6 mm ID, particle size 4 pm, and bonded phase coverage 4.05 pmol/m. Eluent phosphate buffer 37.10 mM KH2PO4 and 4.29 mM Na2HP04 calculated to provide a pH of 6.0. After addition of the desired amount of sodium 1-hexanesulfonate, NaCl was added so that the total sodium concentration was 50 mM (constant ionic strength). Experimental data were fitted by Eq. 8.

It should be remarked that the aim of the PLACID and FLINT technologies is to complement existing battery recycling plants, yielding the best synergy and complementary aspects of both the pyro and hydro lines. 

Figure 2 Chromatograms of plasma (A) and urine (B) from subjecfs supplementing with creatine. The separations were carried out on a Synergi lOp Hydro RP 80 250x4.6mm column using a 20 mmol I potassium phosphate buffer, pH 6.5, for the plasma and 14.7 mmol 1 potassium phosphate-2.3 mmol I tetrabutyl ammonium sulfate, pH 5.0, for the urine, with detection by absorption at 210 nm. For both samples, protein was precipitated out before injection using acetonitrile. The plasma and urine were diluted 5-fold and 87-fold, respecfively, during sample preparation.

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