Symphyti radix

Tittel, G., H. Hinz, and H. Wagner. 1979. Quantitative bestimmung der pyrrolizidinalkaloide in Symphyti radix durch HPLC. Planta Med. 37(1). 

The analysis of pyrrolizidine alkaloids in Radix symphyti was reported by Tittel et al. (18,26). Analysis of the N-oxides could be performed on an octadecyl column (Fig.3.6). The reduced alkaloids could be separated on an alkylamino column. Because of interfering peaks, the latter method was not suitable for the analysis of plant material. The N-oxides could also be separated on an alkylamino stationary phase using acetonitrile - water (98 2) as mobile phase (Fig.3.7)2 . 

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