SWXL columns

I. SILICA-BASED TSK-GEL SW/SWxl COLUMNS FOR GEL-FILTRATION CHROMATOGRAPHY (GFC)... [Pg.93]

Elution Proteins 0.3 M NaCl in 0.1 M (0.05 M for SWxl columns) phosphate buffer, pH 7 dextrans and PEOs distilled water. [Pg.94]

Kato and coworkers recommend the use of 0.05 M sodium phosphate buffer (pH 7.0) containing 0.3 M NaCl to obtain true size exclusion behavior for most proteins on 5 pm TSK-GEL SWxl columns (7). [Pg.89]

The hydrophilic surface characteristics and the chemical nature of the polymer backbone in Toyopearl HW resins are the same as for packings in TSK-GEL PW HPLC columns. Consequently, Toyopearl HW packings are ideal scaleup resins for analytical separation methods developed with TSK-GEL HPLC columns. Eigure 4.44 shows a protein mixture first analyzed on TSK-GEL G3000 SWxl and TSK-GEL G3000 PWxl columns, then purified with the same mobile-phase conditions in a preparative Toyopearl HW-55 column. The elution profile and resolution remained similar from the analytical separation on the TSK-GEL G3000 PWxl column to the process-scale Toyopearl column. Scaleup from TSK-GEL PW columns can be direct and more predictable with Toyopearl HW resins. [Pg.150]

Fig. 3 Purification of the isolated polysaccharide CSP-1 from cultured Cordyceps. (a) Partial purification of polysaccharides from cultured Cordyceps mycelia by DEAE-cellulose chromatography. Extract was loaded onto the DEAE column (3.5x30 cm), and eluted with 0 to 0.5 M NaCI, as indicated by dotted line, in 10 mM Tris-HCI pH 7.4 having a flow rate of 30 mLTh. Rve milliliter fractions were collected, (b) The polysaccharide-enriched fractions were applied onto a Sephacryl S-300 column equilibrated with 0.2 M NaCI in 10 mM Tris-HCI buffer pH 8.0. The first-peak fractions (CSP-1) were collected, (c) HPLC profile of CSP-1 by using TSKgel G3000 SWXL (7.8 mm ID x 30 cm) column. Molecular markers are indicated by arrows in kOa. The polysaccharide profile was detected by refraction index detector. The insert shows the molecular weight determination of CSP-1 (reproduced from ref. [25] with permission from Elsevier)...

$Fig. 3 Purification of the isolated polysaccharide CSP-1 from cultured Cordyceps. (a) Partial purification of polysaccharides from cultured Cordyceps mycelia by DEAE-cellulose chromatography. Extract was loaded onto the DEAE column (3.5x30 cm), and eluted with 0 to 0.5 M NaCI, as indicated by dotted line, in 10 mM Tris-HCI pH 7.4 having a flow rate of 30 mLTh. Rve milliliter fractions were collected, (b) The polysaccharide-enriched fractions were applied onto a Sephacryl S-300 column equilibrated with 0.2 M NaCI in 10 mM Tris-HCI buffer pH 8.0. The first-peak fractions (CSP-1) were collected, (c) HPLC profile of CSP-1 by using TSKgel G3000 SWXL (7.8 mm ID x 30 cm) column. Molecular markers are indicated by arrows in kOa. The polysaccharide profile was detected by refraction index detector. The insert shows the molecular weight determination of CSP-1 (reproduced from ref. [25] with permission from Elsevier)...$

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