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Super-and subcritical fluid chromatography

Terfloth, G. Enantioseparations in super-and subcritical fluid chromatography,... [Pg.249]

Super- and subcritical fluid chromatography (SFC) underwent extremely rapid development in the mid-1980s, and was believed to be a technique with a great potential that combined the advantages of GC and HPLC. However, inherent fundamental and technical limitations of this technique became apparent and led to some stagnation in its further development. [Pg.164]

Another important application of CDs is (chiral) drug analysis. In this field, CDs are used in combination with UV-VIS spectrometry, fluorescence and NMR spectroscopy, mass spectrometry, in electrochemical analysis and most widely in various separation techniques such as thin-layer chromatography (TLC), gas chromatograpy (GC), high-performance liquid chromatography (HPLC), super- and subcritical fluid chromatography (SFC) [13] and capillary electrophoresis (CE) [9], [16]. [Pg.158]

Anton K, Eppinger J, Frederiksen L, Francotte E, Beiger TA, Wilson WH (1994) Chiral separations by packed-column super- and subcritical fluid chromatography. J Chromatogr A 666 395 01... [Pg.200]

There are many classes of CSPs applicable in different mobile-phase modes. In particular, CSPs based on derivatized polysaccharides, native and derivatized cyclodextrins, macrocyclic glycopeptides, and Pirkle-type chiral selectors operate quite well in four separation modes, i.e RP, polar organic phase, NP, and super- or subcritical fluid chromatography (SFC) conditions. It is common that a chiral compound can be separated on the same CSP in more than one separation mode [58, 160, 166, 170-176]. For example, Nutlin-3, a small molecule antagonist of MDM2, has been baseline resolved from its enantiomer in all four mobile-phase conditions (Fig. 16) [170]. Multimodal enantioseparation on the same CSP would be greatly beneflcial for chiral method development in pharmaceutical industry. [Pg.182]

The general confusion about the physical and chemical meaning of the words super and critical has lead to a plethora of terms such as subcritical fluid chromatography, near-critical fluid chromatography, and enhanced fluid chromatography to deal with the fluids when they are nearly but not quite super critical. All these names, including SFC, actually describe the same technique. [Pg.4572]

Supercritical fluid chromatography (SEC) refers to the use of mobile phases at temperatures and pressures above the critical point (supercritical). SEC uses carbon dioxide as a main component in the mobile phase because its critical point (31.3°C, 7.39 MPa) is easy to reach. However, carbon dioxide is similar to alkanes in solvent strength and therefore unsuitable for the elution of polar compounds. This character is corrected by the addition of a significant amount of polar solvents, mainly alcohols, to increase the polarity of the mobile phase. In such conditions, the supercritical state is not actually reached. Often temperatures lower than the critical and pressure above the critical are applied. These are designated as subcritical conditions. Nevertheless, separations are performed indistinctively in super- or subcritical conditions. [Pg.1619]


See other pages where Super-and subcritical fluid chromatography is mentioned: [Pg.175]    [Pg.176]    [Pg.198]    [Pg.86]    [Pg.175]    [Pg.176]    [Pg.198]    [Pg.86]    [Pg.121]    [Pg.154]    [Pg.419]    [Pg.1390]   


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Subcritical fluid chromatography

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