Vectors suicide

Shortly after this, other workers also described suicide vectors using the hok gene under the control of the /ac promoter (Bej, Perlin Atlas, 1988 Atlas etal., 1989)-They found that when cells were grown without selective pressure for the plasmid... 

Bej, A. K., Perlin, M. Atlas, R.M. (1988). Model suicide vector for containment of genetically engineered microorganisms. Applied and Environmental Microbiology, 54,2472-7. 

This approach is based on the use of suicide vectors that express a toxic gene in response to viral regulatory proteins such as Tat and/... 

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