12 - substrates high-throughput

For solar cell applications, thin film LPE is economically viable only if it is combined with a low-cost multicrystalline Si substrate (high-throughput silicon ribbons, upgraded metallurgical grade silicon MG-Si) or with a foreign substrate (glass, ceramic, metallic sheet... see section 9-7). 

Figure 3.17 Computational high throughput screening for 736 pure metals and surface alloys. The rows indicate the identity of the pure metal substrates, and the columns indicate the identity of the solute embedded in the surface layer of the substrate. The solute coverage is (a) ilVIL, (b) ML, and (c) 1 ML, and the adsorbed hydrogen coverage is also jML. The diagonals of the plots correspond to the hydrogen adsorption free energy on the pure metals. Adapted from [Greeley et al., 2006] see this reference for more details.

Yang, Y. Z., Babiak, P. and Reymond, J. L. (2006b). Low background FRET-substrates for lipases and esterases suitable for high-throughput screening under basic (pH 11) conditions. Org. Biomol. Chem. 4,1746-1754. 

In a direct immunoassay the immobilized antibody binds to the corresponding antigen. The competitive immunoassay relies upon the competition of the analyte with a labelled analyte for antibody binding. These formats are widely used for high throughput affinity arrays. A sandwich immunoassay is based on the trapping or capture of the analyte by another antibody. In ELISA (enzyme linked immunosorbent assays) the second antibody is conjugated with an enzyme. The bound enzyme labelled antibody is detected by its ability to break down its substrate to a colored product. 

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