Submitochondrial particles and

Argese, E., C. Bettiol, A.V. Ghirardini, M. Fasolo, G. Giurin, and P.F. Ghetti. 1998. Comparison of in vitro submitochondrial particle and Microtox assays for determining the toxicity of organotin compounds. Environ. Toxicol. Chem. 17 1005-1012. 

Gustavson, K.E., Svenson, A., and Harkin, J.M. Comparison of toxicities and mechanism of action of / -alkanols in the submitochondrial particle and the Vibrio fischeri bioluminescence (Microtox ) bioassay. Environ. Toxicol. Chem., 17(10) 1917-1921, 1998. 

The thiocyanate ion-selective electrode [21,247] This has a heterogeneous membrane containing AgCNS. It is used for the determination of the activity of SCN in suspensions of submitochondrial particles and chromatophores from Rhodospirillum rubrum in order to measure the membrane potential of these particles [200], as well as for other applications. 

As stated above, submitochondrial particles and complex I exhibit both NADPH dehydrogenase and NADPH-to-NAD transhydrogenase activities. These activities have similar pH dependencies (Fig. 15) and are... 

The rate with the quinone is less than 1% of the rate with TMPD so that it would be interesting to determine whether the in-vivo rate of quinone turnover during succinate oxidation is consistent with the operation of the electron transport system. Although the Sulfolobus enzyme is composed of four subunits, there is no evidence that all are associated with the enzyme other than that they copurify with enzyme activity. The succinate dehydrogenase from eucarya and bacteria have two subunits whose M, are approximately 70000 and 28 000 [112]. They appear to be analogous to the M, 66000 and 31 000 subunits from the Sulfolobus succinic dehydrogenase. The M, 66 000 subunit from Sulfolobus is catalytically active by itself, and like the Mr 70000 subunit contains covalently-bound FAD. Antisera against the Mr 66000 Sulfolobus subunit cross-reacts with an Mr 67 000 constituent in membranes from T. acidophilum, S. solfataricus, beef-heart submitochondrial particles, and Bacillus subtilis. 

Studies with beef-heart submitochondrial particles initiated in Green s laboratory in the mid-1950s resulted in the demonstration of ubiquinone and of non-heme iron proteins as components of the electron-transport system, and the separation, characterisation and reconstitution of the four oxidoreductase complexes of the respiratory chain. In 1960 Racker and his associates succeeded in isolating an ATPase from submitochondrial particles and demonstrated that this ATPase, called F, could serve as a coupling factor capable of restoring oxidative phosphorylation to F,-depleted particles. These preparations subsequently played an important role in elucidating the role of the membrane in energy transduction between electron transport and ATP synthesis. 

Since bacterial membranes, like submitochondrial particles, oxidize NADH in a ratio NADH/O equal to 1, it follows that / in this particular case will be 0.00389/0.00251 = 1.55. Table III shows the dependence of the correction factor on pH and temperature for respiratory enzymes of rate heart submitochondrial particles and membrane preparations from Citrobacterfreundi, when Hb02 or HbCPA were used as oxygen donors. It... 

Experimental synthesis of ATP at the octane/water interface was carried out [11, 12, 20]. The proton flow through the ATP-synthetase complex from octane to water was provided by creating an excess (relative to equilibrium) concentration of undissociated (or Lewis) acid in the octane phase (Fig. 37). This was achieved in three ways by direct addition of add-pentachlorphenol to octane, by the action of NADH-ferricyanidreductase of the respiratory chain of submitochondrial particles, and also through the action of the H -pump of bacteriorhodopsin sheets from Halobacterium halobium. 

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