Structures of Cytochrome P450 Enzymes

Departments of Molecular Biology and Biochemistry, Pharmaceutical Sciences, and Chemistry, 2206 Natural Sciences 1, Mail Code 3900, Urriversity of California, Irvine, CA 92697-3900, USA e-mail poulos uci.edu 

Department of Molectrlar and Experimental Medicine, The Scripps Research Institute, 10550N, Torrey Pines Rd., La Jolla, CA 92037-1000, USA e-mail johnson scripps.edu 

Ortiz de Montellano et al. (eds.). Cytochrome P430, DOI 10.1007/978-3-319-12108-6 1 Springer International Publishing Switzerland 2015 

In contrast to prokaryotic P450s, eukaryotic P450s ate generally membrane-bound proteins. Most eukaryotic P450s are incorporated into the endoplasmic reticulum. However, several mammalian P450s that participate in the synthesis of 

Recently, additional evidence for the hehcal nature of the TMH was obtained from sohd-state nuclear magnetic resonance (NMR) studies of rabbit microsomal CYP2B4 incorporated 

---

Poulos TL, Johnson EF. Structures of cytochrome P450 enzymes. In Cytochrome P450 Structure, Function, Genetics. 3rd edition. Ortiz de Montellano PR, ed. 2005. Kluwer Academic/Plenum Publishers, New York. pp. 87-114. 

Structures of Cytochrome P450 Enzymes Fluorescent Reporter... 

Fig. 25.5. General structure of cytochrome P450 enzymes. O2 binds to the P450 Fe-heme in the active site and is activated to a reactive form by accepting electrons. The electrons are donated by the cytochrome P450 reductase, which contains an FAD plus an FMN or Fe-S center to facilitate the transfer of single electrons from NADPH to O2. The P450 enzymes involved in steroidogenesis have a somewhat different structure. For CYP2E1, RH is ethanol (CH3CH2OH) and ROH is acetaldehyde (CH3COH).

Shaik, S., Kumar, D., de Viser, S. R, Altun, A., 8c Tiel, W. (2005). Theoretical perspective on the structure of cytochrome P450 enzymes. Chemical Reviews, 105, 2279. 

---