Structural radial chromatography

The diastereomeric ratios were determined by examining the H-NMR spectra of the crude reaction mixture and the diastereomers were easily separated by radial chromatography. The structures are determined on the basis of both NOESY H-NMR and 13C-NMR data. 

Chemical methods As with many areas of natural product chemistry, new impetus in the chemistry of lichen substances is provided by the more rapid and improved methods for detecting, isolating and purifying these compounds and in determining their structure. The techniques of preparative TLC, radial chromatography and preparative HPLC provide rapid and efficient methods for the purification of lichen substances and developments in mass spectrometry, proton and carbon-13 nuclear magnetic resonance (NMR) spectroscopy and X-ray analysis greatly aid structural smdies. 

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