Strong cation-exchange chromatography

Essader, A.S., Cargile, B.J., Bundy, J.L., Stephenson, J.L., Jr. (2005). A comparison of immobilized pH gradient isoelectric focusing and strong-cation-exchange chromatography as a first dimension in shotgun proteomics. Proteomics 5, 24—34. 

Vollmer et al. [33] studied human nucleolus proteome using off-line strong cation exchange chromatography and microfraction collection combined with... 

In the gel-free approaches, also referred as shotgun proteomics, proteins in a mixture are digested and the peptides released separated by reverse-phase HPLC, either alone or combined with strong cation exchange chromatography... 

Figure 5 High-performance strong cation-exchange chromatography of ephedrine alkaloids and synephrine in dietary supplements. UV and fluorescence chromatograms of (A) standard solution ( 3 ng per component, except 1.6 tg of component 1), (B) Xenadrine RFA-1, (C) Xetalean, and (D) Ultra Diet Pep. Analytes 1, ( )-synephrine 2, (-)-norephedrine 3, (+)-norpseudoephedrine-HCI 4, (-)-ephedrine-HCI 5, (+)-pseudoephedrine 6, (-)-A/-methylephedrine 7, (+)-N-methylpseudoephedrine. Reproduced from R. A. Niemann M. L. Gay, J. Agric. Food Chem. 2003, 51, 5630-5638.

Monitoring Effect of Polypeptide Chain Length and Charge Density on Peptide Elution Behavior During Strong Cation-Exchange Chromatography... 

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