Strictosidine glucosidase

STEVENS, L.H., BLOM, T.J.M., VERPOORTE, R., Subcellular localization of tryptophan decarboxylase, strictosidine synthase and strictosidine glucosidase in suspension cultured cells of Catharanthus roseus and Tabemaemontana divaricata. Plant Cell Rep., 1993,12, 563-576. 

Fig. 8.1 Sequence of reactions and pathways involved in the biosynthesis of indole alkaloids in Catharanthus roseus. The dotted lines indicate multiple and/or uncharacterized enzyme steps. Tryptophan decarboxylase (TDC), Geraniol Hydroxylase (GH), Deoxyloganin synthase (DS), Secologanin Synthase (SLS) Strictosidine synthase (STR1), Strictosidine glucosidase (SG), Tabersonine-16-hydroxylase (T16H), Tabersonine 6,7-eposidase (T6,7E), Desacetoxyvindoline-4-hydroxylase (D4H), Deacetyl-vindoline-4-O-acetyltransferase (DAT) and Minovincinine-19-O-acetyltransferase (MAT) represent some of the enzyme steps that have been characterized.

Figure 2.9 Enzymic formation of ajmalicine. TDC, tryptophan decarboxylase STS, strictosidine synthase STG, strictosidine glucosidase POD, peroxidase.

Gerasimenko I, Sheludko Y, Ma X, Stockigt J. Heterologous expression of a Rauvolfia cDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids. Eur. J. Biochem. 2002 269 2204-2213. 

From strictosidine, the first step toward the various types of indole alkaloids is deglucosylation by strictosidine glucosidase (SG, EC 3.2.12). The key to the diversification in indole alkaloids must be at the glucosidase or in the steps directly after this enzyme. The glucosidase and the subsequent steps have thus been the subject of quite a few studies. 

T. J. C. Luijendijk, Strictosidine glucosidase in alkaloid biosynthesis. Ph.D. Thesis, Leiden University, 1995. 

Figure Simplified scheme for the biosynthesis of the antiar- > rhythmic agent ajmaline. Only the key steps are shown in the scheme. The cofactors given on the left side participate in individual enzymatic reactions. The following enzymes were identified in the 15-step sequence leading to the final product ajmaline 1 strictosidine synthase 2 strictosidine glucosidase ...

Strictosidine synthase 2 strictosidine glucosidase 3 cathenamine synthase... 

Fig. 8.5 Compartmentation of alkaloid biosynthesis in Cathamnthus roseus. AS anthianilate synthase, CR NADPH cathenamine reductase, DAT deacetylvindoline 17-O-acetyltransferase, ER endoplasmic reticulum, GlOH geraniol 10-hydroxylase, GAP glyceraldehyde-3-phosphate, NMT S-adenosyl-L-methionine methoxy-2, 16-dihydro-16-hydioxylagersonine-lV-methyltransferase, OHT desacetoxyvindoline-4-hydroxylase, SGD strictosidine -glucosidase, STR strictosidine synthase TDC tryptophan decarboxylase, THAS NADPH tetrahydroalstonine reductase (Adopted from Ref. [10])... 

Barleben, L. et al. (2007) Molecular architecture of strictosidine glucosidase the gateway to the biosynthesis of the monoterpenoid indole alkaloid family. Plant Cell 19,2886-2897... 

Sun, J. and Peebles, CA. (2015) Engineering overexpression of ORCA3 and strictosidine glucosidase in Catharanthus roseus hairy roots increases alkaloid production. Protoplasma.,... 

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