Storage of Tissue Slides

It has also been demonstrated that the staining of p53 in mammalian ductal carcinoma decreased after slides were stored for 2 months at room temperature, but the antigen loss 

There are many reasons for the lack of consensus on this highly complex phenomenon, and they are discussed below. Various studies mentioned above were conducted using different parameters of antigen retrieval methods, including antigen retrieval fluids, pH, heat source, temperature, and duration of treatments for detecting different antigens. The type of fixation and duration of fixation also varied in these studies. Other variants were the type of epitope and antibody and source of antibodies used. 

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