Sterilized Grain from Agar Media

Inoculation of Sterilized Grain from Agar Media 

Once fully colonized, these grain masters are now used for the further production of grain n p,spawnlngf on/jgallorPontalners.MastersmusrbetransferredwIthlnafewdaysofThelrfullcol-spawn otherwise the mycellated kernels do nof break apart easily. A step by step description of 

Once completed, SET THE SPAWN JARS ON A SEPARATE SHELF AND WAIT TWELVE TO TWENTY-FOUR HOURS BEFORE USING. ThiswaiTing period is impor-tanT because some of The spawn may not recover, suffering usually from bacterial contamination. Had these jars been used, the contamination rate would have been multiplied by a 

Inspect the jars again for signs of contamination. After twelve to twenty-four hours, the mycelium shows signs of renewed growth. 

If the masters had been shaken the night before, the inoculations can begin the following morning or as soon as the receivingjars (G-2) have cooled. Again, wash the lab, be personally clean and wear newly laundered clothes. 

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