Stereospecificity in Other Dehydrogenases

dehydrogenases that utilize the other side of the nicotinamide ring were found. These enzymes are, for example, 3-hydroxy-steroid dehydrogenase from Pseudomonas testosterone (TsLlailsLy et al. 1955, Jarabak and Talalay 1960), the pyridine nucleotide transhydro-genase from Pseudomonas fluoresoens (San Pietro et al. 1955), and glycerol-3-phosphate dehydrogenase from muscle (Levy and Vennesland 1 957).  

NAD+-dependent dehydrogenases are subdivided into two classes. Dehydrogenases that exhibit the same specificity as ADH 

Prior to the investigation by Cornforth et al. (1962), it was not clear whether or Hg corresponds to Using horse liver alcohol 

The optical rotatory dispersion (ORD) curves of the succinic acids, which were believed to be enantiomers of each other, gave a good contrast. The ORD spectrum of the succinic acid-2- obtained from the reduction with NADH-4-d was proved to be identical with 

Therefore, it was concluded that the absolute configuration at the 4 position of NADH-4-d whose deuterium was introduced from the substrate on HLADH is This means that the H can be depicted 

---