Steps involved in the ASE process

Filling and pressurization of the extraction cell are done once the cell is loaded and tightened. Extractions can be performed either by preheating the cell before filling with 

High-pressure, high-temperature solvent extraction 

Heating of the cell at the selected temperature for equilibration at a constant pressure is done in aceordanee with the temperature to be used in the extraction and with how rapidly the oven ean reach it. Usually, 5 min suffices to equilibrate the system. 

Static extraction is developed after filling the cell with solvent and reaching the preset temperature and pressure. During it, the analytes are released from the sample to the extractant. 

The transfer step begins immediately after the static extraction step finishes. The pressure valve is opened and the solvent is allowed to flow to the collection vial. Cooling the vial is probably unnecessary as it appears to result in no differenee in terms of recovery or precision. A filter can be placed in the bottom of the extraction cell to avoid collecting suspended powders in the vial [30,73]. 

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