Starter calibrations

A commonly used method development strategy is to develop starter calibrations that can be used to find unrepresented sample and process variation (1). 

The molecular weight data were obtained from integrating SEC traces between 15.5 and 26.1 min (i.e., monomer peak was excluded), and calibrated on the basis of polystyrene standards (the calibrated molecular weight of starter sequences 60 and 66 is ca. 1.5 kDa the molecular weights of polymers were underestimated). 

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