Staphylococcus nuclease

Figure 2.15 The Greek key motif is found in antiparallel p sheets when four adjacent p strands are arranged in the pattern shown as a topology diagram in (a). The motif occurs in many p sheets and is exemplified here by the enzyme Staphylococcus nuclease (b). The four p strands that form this motif are colored red and blue.

The recombinant strain expressed the Staphylococcus nuclease directed to the periplasm without affecting PHA production. During downstream processing, the viscosity of the recombinant lysate was significantly reduced to facilitate the subsequent purification steps. 

Staphylococcus nuclease ISNC 1.65 0.161 1 Catalytic Loll and Lattman (1989)... 

Staphylococcus nuclease Subtilisin Carlsberg Hydrolysis of nucleic acids, Ca2+ at active site403... 

Figure 26 shows the X-ray structure of Staphylococcus nuclease (SNase) consisting of three 7-helices and a five-stranded /i-barrel, with a total of 149... 

In summary, various experimental data, especially with systematic mutations of Staphylococcus nuclease and human thioredoxin, support that the hydration dynamics makes the dominant contribution to the total solvation response... 

Staphylococcus nuclease is an enzyme that catalyzes the hydrolysis of DNA. The overall hydrolysis reaction is as follows ... 

The transport of calcium would also seem to involve phosphorylated proteins— for example, a phosphorylated protein carries calcium in the blood stream. The binding constant of these centers for Ca — 10 ) and lanthanides — 10 ) indicates that there are probably two (or three) more anionic, carboxylate(P), groups as well as the one phosphate at the binding center. This is certainly the case in the staphylococcus nuclease (44). There are also four carboxylate residues in the terminal sequence of trypsinogen, which is activated by calcium. 

Figure 3.17 Signal from mass spectrometry of staphylococcus nuclease (SNase).

A mass spectrum of the protein staphylococcus nuclease (SNase) (Figure 3.17) is to be decomposed by the Daubechies-3 wavelet transform at three levels and approximation Aj as well as details Dj -Dj are to be evaluated. 

Staphylococcus nuclease is an enzyme that catalyzes the hydrolysis of DNA. 

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