Standard affinity interpretation

The results obtained by measuring the affinity to oxygen in the presence of various monohydric alcohols (methanol, ethanol, 2-propanol, 1-propanol) 140-144> were interpreted in terms of the Monod-Wyman-Changeux model145), by which the change of the standard free-energy difference between R and T state in the absence of oxygen, due to the addition of alcohol, can be determined, i.e. 

Affinity constants KAg and Kl (KAg = ka[/kdl Kl = ka>2/kd>2) will differ between the free analyte and the labelled analyte (Tijssen, 1985), especially when the label is an enzyme. In addition, the analyte (of the standard or sample) competes with a derivative, not with the identical structure, and the difference in recognition between the two will have an effect. And it will also differ when the (derivative of the) analyte is bound to the surface via a protein. All these facts have to be taken into consideration when interpreting the results. 

Various Physical Properties.—Radical cations of 2,5-dialkylthiothiophens have been obtained and their e.s.r. spectra interpreted in terms of unequally populated rotational isomers. The mass spectra of some simple thiophens and some azines derived from 2-acetyl- and 2-formyl-thiophens have been obtained. Molecular ion isomerizations occurring in 3-phenylthiophen and two brominated derivatives have been followed in both slow and fast reactions using C-labelling. Affinities, standard heats, and standard entropies of dyeing of some thiophen-containing azo dyes have been... 

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