Staining with uranyl acetate negative

Figure 6.13 Electron micrograph of human spleen ferritin viewed at a magnification of x 170 000 after negative staining with uranyl acetate. Both the darkly coloured iron-rich cores and the clear-coloured protein shells are clearly visible. (From Crichton, 1991.)...

Fig. 19 TEM image of toroidal micelles from a PAA-PMA-PS triblock copolymer (A). This sample was cast from a solution with 0.1 wt% PAA99-PMA73-PS66 triblock copolymer, a THF water volume ratio of 1 2, and an amine acid molar ratio of 0.5 1 by addition of 2,2-(ethylenedioxy)diethylamine. The cast film was negatively stained with uranyl acetate. A schematical representation of theses micelles is also shown (B). Reprinted with permission from [279], Copyright (2004) American Association for the Advancement of Science...

Fig. 2. Electron micrographs of negatively stained preparations of E. coli pyrophosphatase. (a and b) Particles stained with sodium silicotungstate (4 g/100 ml, pH 7) the particles in (b) were first fixed in glutaraldehyde (05 g/100 ml). (c) Non-fixed particles stained with uranyl acetate (2 g/100 ml, pH 4).

Swollen fibers of chromatin from the nucleus of the chicken red blood cell. The electron micrograph is enlarged about 325,000x and negatively stained with uranyl acetate. (Micrograph courtesy of A. L. Olins and D. E. Olins.)... 

Figure 3. Electron micrograph of cellulose from beechwood, boiled with 95% TFA for 8 hr. Negative staining with uranyl acetate.

Figure 9.2 Transmission electron micrograph of Cowpea mosaic virus particles negatively stained with uranyl acetate. The scale bar is lOOnm.

Fig. 7 TEM image of aggregates of PAELi-fc-PLPheg negatively stained with uranyl acetate specimen was prepared by deposition of a drop of a 0.2 wt % polymer solution on a carbon-coated copper grid, drawing-off the solution with filter paper, and subsequent drying in vacuo. Reprinted with permission from [42], copyright (1997) Hiithig Wepf...

Figure 17. The vitelline envelope receptor for lysin (VERL) is a giant glycoprotein. (Left panel), electrophoresis of VERL on 2.5% acrylamide gels (silver staining) shows it resolves as two sharp bands between titin (2,800K) and nebulin (770K), Lane 1, rabbit muscle extract myosin (205) lanes 2-6, different loads of pink abalone VERL resolved into two components. (Right panel), electron micrography of VERL molecules negatively stained with uranyl acetate. The VERL fibers are 13 nm in diameter (from Swanson and Vacquier, 1997).

Figure 1.5.12 Typical electron micrograph of a bilayer vesicle membrane negatively stained with uranyl acetate (black regions). The white membrane corresponds to the hydrophobic lipid bilayer. Its width is about 40 A.

Fig. 22. Transmission electron micrographs of tunicin. (A) Microciystals negatively stained with uranyl acetate. (B) Ultiathin section of microfibrils in bright field. (C) Transmission election miciographs of cellulose miciociystals n atively stained with uranyl acetate. (D) Ultiathin section of a cotton fiber in bright field. (E) Transmission electron micrographs of parenchyma cellulose miciofibrils n atively stained with uranyl acetate. (F) Ultiathin section of microfibrils in bright field. (G) X-ray diftiaction diagrams of cellulose microoystal (a) tunicin cellulose (b) cotton and (c) parenchyma. ...

Fig. 3 SC piece from an insect spermatocyte Laplatacris sp.), negatively stained with uranyl acetate. The central element (asterisk) is scalariform. Individual transverse fibers (arrowheads) are observed. The lateral elements show a granular structure. X290,000.

Figure 2.3 The appearance of T4 phage after negative staining with uranyl acetate (a) and in the frozen, hydrated state (b). Note the reversal of contrast between (a) and (b) [Micrograph (a) is courtesy of Dr Naiqian Cheng]...

E) Electron microscopy of the PSI complex Specimens for electron microscopy were prepared and negatively stained with uranyl acetate as described by Ford and Holzenburg [6]. The long and short axis of 20 particles were measured and mean values of 14.8 nm and 9.1 nm respectively, were obtained. 

Figure 9 The Pp phase of bovine brain sphingomyelin revealed by negative staining with uranyl acetate on (A) a multilammelar body and (B) a single bIlayer. Liposome particles are present on the surface of the larger lipid bilayer structures. Bars = 100nm. (Reproduced with permission from Harris JR (1986) Micron and Microscopica Acta 17 175-200.)...

Samples (6.9 x 10 M) WCTe negatively stained with uranyl acetate (2 wt% in water) for observation by TEM. ... 

Analysis under a transmission electron microscope by negative staining with uranyl acetate showed stable vesicle formation of nido-carborane lipid 2. The m do-carborane lipid 2 (CL) was incorporated into DSPC liposomes in a concentration-dependent manner (Nakamura et al., 2004). 

FIGURE 6.12 A transmission electron microscopic image of single F-actin filaments. The filaments are negatively stained with uranyl acetate. 

Figure 10.2-1. Electron microscopy to image gene transfer structures. In this example, the authors have imaged the gene transfer structure they developed (human papillomavirus-like particles) after uranyl acetate negative staining. Reprinted from Ref. 7, with permission from Oxford Journals.

For a high contrast in the electron micrographs, the sample was stained negative with uranyl acetate. This leads to denaturation of the enzyme, and this might be connected with considerable changes of shape and structure of the protein. In order to avoid these problems, cryo-electron microscopy of frozen-hydrated samples has been applied, similar to that carried out with FqFj from E.coli (6). 

Plasmid harboring the mutated gene for Rubisco was used to transform E.coli cells. Rubisco was isolated from cells grown on 2YT medium under selective conditions, and purified as previously described (5). Analysis of the mutant protein involved analytical gel filtration, electron microcopy of negatively stained samples with uranyl acetate. The zymatic capability was assayed by RuBP dependent fixation of C02 (8). The extent of activation of the enzyme by CO and Mg, and the ability to bind phosphorylated ligands were tested using 2-carboxy arabinitol bisphosphate, CABP (9). 

Negatively-stained PBS with uranyl acetate (Lundell et al., 1981) were observed with electron microscope. 

---