Staining of Proteolipids, Lipids, and Lipoproteins

5 g Sudan Black B (Solvent Black 3, Fat Black HB, C.1.26150) is dissolved in 20 ml acetone, and then 15 ml of glacial acetic acid are added followed by 80 ml ddH20. The solution is centrifuged for 15 min at RT with 3000 x g, the supernatant is used for staining. 

After electrophoresis the gel is fixed either in the common acetic acid/methanol/H20 mixture or in Soln. B, but never in aqueous 

Staining is done by incubation with a tenfold gel volume of Soln. A overnight. Use several changes of Soln. B for destaining. Remaining colloidal dye on the surface of the gel is wiped off with a wetted tissue. 

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