Stachyose from verbascose

Kandler and coworkers818 isolated from the mature seeds of V.faba (see also, Refs. 293 and 298) an enzyme that transfers the D-galactosyl group of galactinol to raffinose, yielding stachyose (compare with Refs. 34, 296, and 615), and to stachyose, yielding verbascose. When... 

Structure. Cryoscopic molecular weight values indicate that verbascose is a pentasaccharide 169). From methylation studies, Murakami concluded that verbascose was a D-galactosyl-substituted stachyose (at C-6 of the terminal D-galactose unit of stachyose). Hot dilute acetic acid removes the D-fructose unit from verbascose to give a tetrasaccharide verbascotetraose (m.p. 240°C. [a] +191.4 (water)). This tetrose has been partially hydrolyzed, with removal of D-glucose, to give the crystalline trisaccharide galactotriose 164)-... 

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