Stabilized plasmid-lipid particles

Figure 6 Encapsulation of plasmid DNA (pDNA) in small sterically stabilized liposomes [stabilized plasmid-lipid particles (SPLP)] using a detergent dialysis procedure. (A) Entrapped pDNA-to-lipid ratio as a function of the initial pDNA-to-lipid ratio (mg/mg). The initial lipid concentration was lOmg/mL. (B) Cryo-electron micrograph showing the structure of SPLP. The location of the plasmid is indicated by the striated pattern superimposed on the liposomes. The bar represents 100 nm.

Figure 7 Pharmacokinetic properties and in vivo gene expression of stabilized plasmid-lipid particles (SPLP). (A) The levels of intact plasmid DNA (pDNA) in the circulation resulting from IV injection of naked plamid pDNA ( ), lipoplexes (O), and SPLP ( ) were determined by Southern blot analysis of plasma samples (100 pg pDNA/mouse). (B) Transgene expression at a distal tumor site resulting from rv injection of naked plamid pDNA ( ), plamid pDNA-cationic liposome complexes (O), and SPLP ( ).

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