SPR kinetics

Fig. 13. (A) Schematic representation of the interaction between neighboring residues of the His-tag (6 consecutive Histidine residues) and an NTA-complexed Ni2+ ion. (B) The performance of LHCII immobilization via chelating interaction. SPR kinetic curve of LHCII immobilization and regeneration cycles, monitored with an Nd YAG DPSS laser (X = 473 nm). (C) Surface plasmon field-enhanced fluorescence emission spectrum of surface attached LHCII compared with the fluorescence emission from free LHCII in solution, excited by an Nd YAG DPSS laser (X — 473 nm).

Fig. 15 SPR kinetics of the incorporation of cytochrome c oxidase (CcOX) into a plasma-polymer supported membrane...

Time differential SPR kinetic curve and cyclic voltammogram.63... 

Simultaneous observation of the SPR kinetic curve and scattered light kinetic curve. 65... 

Figure 5 shows the simultaneous observation of a SPR kinetic reflectivity curve and a cyclic voltammogram during the electropolymerization from the 1st to 5th cycle of 0.1 M aniline in 0.5 M HCl solution at a scan rate of 20 mVs. ... 

Fig. 5 Simultaneous observation of a SPR kinetic curve and a cyclic voltammmogram during the...

Iwasaki et al. reported that by using the simple system of time differential SPR data (A0/At), a Faradaic current behavior can be observed based on the same potential dependence behavior with the voltammetric currents [22]. Figure 6 shows the time differential SPR kinetic... 

Fig. 6 The time differential SPR kinetic curve (dR/dt) as a function of potential cycling between -0.2 and 1.0 V at a scan rate of 20 mVs and the cyclic voltammogram of 1st cycle (a), 2nd cycle (b) and 5th cycle (c).

However, there is no relation below ca. 0.4 V between the cyclic voltammograms and time differential SPR kinetic reflectivity curves in all cycles. To further probe this behavior, we measured the scattered light intensity enhanced by SPR from the polyaniline/electrolyte interface during the electropolymerization. Figure 7 shows the angular dependence of the measured reflectivity and scattered light intensity from the substrate/gold/electrolyte system before the electropolymerization. 

As shown in Fig. 8, an obvious increase of the scattered light intensity was observed below ca. 0.4 V at every cycle, and the SPR kinetic reflectivity curve also increased in this region at every cycle. Two possible explanations can be considered. 

Keywords SPR Kinetics self-assembly metal phosphonate film peryleneimide... 

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